rAPid Alkaline Phosphatase

Descrição detalhada : rAPid Alkaline Phosphatase is an alkaline phosphatase isolated from bovine intestine and supplied as a recombinant enzyme of 56 kD, expressed in the yeast, Pichia Pastoris. The recombinant form ensures consistency and safety. rAPid Alkaline Phosphatase catalyzes the dephosphorylation of 5' phosphates from DNA and RNA, nucleotides, and proteins. Alkaline phosphatase treatment prevents self-ligation of DNA fragments by removing the 5'-phosphoryl termini required by ligases; this feature is of major importance in cloning strategies to decrease vector background. Unlike calf intestinal phosphatase, rAPid Alkaline Phosphatase is rapidly, completely, and irreversibly inactivated by heat treatment for two minutes at +75 C. It is therefore, an excellent alternative to Shrimp Alkaline Phosphatase. In addition, the enzyme is active in restriction enzyme buffers. Therefore, restriction enzyme digestion, dephosphorylation, enzyme inactivation, ligation, or 5'-end labeling can be performed without purification steps.
Sinónimos : alkaline phosphatase, cloning of dna fragments
Armazenamento : -20C
Embalagem : 1X1EA