Ready-to-Assay LH Glycoprotein Hormone Receptor Frozen Cells

Descrição detalhada : EMD Millipore's Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. EMD Millipore's ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.Luteinizing Hormone (LH), also known as lutropin, is produced by the pituitary gland and is critical for fertility and reproduction. In women, LH modulates ovulation. In men, LH stimulates testosterone production. Several naturally occurring mutations in the LH receptor gene have been associated with human reproductive disorders (Themmen et al.). Together with TSH, hCG and FSH, LH is a member of the glycoprotein hormone family. Each member shares a common alpha subunit, whereas the beta subunit confers functional specificity. Upon activation, LH signals primarily through the Galphaqs/adenylyl cyclase/cAMP/PKA pathway as has been shown in native granulosa cells. EMD Millipore's cloned human LH receptor-expressing ChemiBrite cells were made by recombinant transfection of HEK293 cells with ChemiBrite clytin, the LH receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. The cells have been cryopreserved at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent, fluorescent and cAMP accumulation analysis of agonists, antagonists and modulators at the LH receptor.
Armazenamento : -80°C
Embalagem : 1X1EA