TBE buffer
REF : 1061772500
Marca : Millipore
Descrição :TBE buffer 10 x pH 8.3 tris-borate-EDTA buffer
Clique para mais informações
Clique para mais informações
Categoria : Biochemistry
Descrição detalhada : Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg2+ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.
Armazenamento : 15-25C
Embalagem : 1X1EA
Armazenamento : 15-25C
Embalagem : 1X1EA
