beta-Secretase Substrate IV, Fluorogenic – Calbiochem

Descrição detalhada : A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the beta-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by beta-secretase results in fluorescence enhancement. Reported Kcat/km values (min-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37C)., A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the beta-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by beta-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.
Sinónimos : beta-Secretase Substrate IV, Fluorogenic - Calbiochem; FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
Fórmula molecula r: C91H129N25O25S
Armazenamento : -20C
Embalagem : 1X1MG