Human iPS Reprogramming Boost Supplement

Descrição detalhada : Small molecules targeting specific signaling pathways have been shown to enhance the efficiency and/or replace the transcription factors required for reprogramming. EMD Millipore has screened multiple chemical compounds and selected the compounds in this kit based on their effects on enhancement of reprogramming efficiency as assessed by the number and overall quality and flat 2D morphology of human iPS colonies generated. The Human iPS Reprogramming Boost Supplement contains two proprietary small molecules in amounts sufficient to supplement over 250 mL human ES/iPS cell maintenance medium. When used in conjunction with the Human STEMCCA lentivirus reprogramming kits (SCR544, SCR545), the Human iPS Reprogramming Boost Supplement enhanced the efficiency of human iPS colony formation over ten-fold and reduced the time to establish full reprogrammed colonies by nearly 50%. The colonies formed possessed the distinctive flat 2D morphology that are reminiscent of normal human ES cell colonies and could be easily passaged. Full reprogrammed human iPS colonies that are SSEA-4-positive, TRA-1-60-positive and Hoechst-Dim are readily established in approximately 20 - 25 days.
Armazenamento : -20°C
Embalagem : 1X1EA