Mouse iPS Reprogramming Boost Supplement

Descrição detalhada : Small molecules targeting specific signaling pathways have been shown to enhance the efficiency and/or replace transcription factors involved in somatic cell reprogramming. EMD Millipore has screened multiple chemical compounds and selected the compounds in this kit based on their effects on enhancement of reprogramming efficiency as assessed by the number and overall quality of mouse iPS colonies generated.The Mouse iPS Reprogramming Boost Supplement contains three proprietary small molecules in amounts sufficient to supplement over 250 mL mouse ES/iPS cell maintenance medium. When used in conjunction with the Mouse STEMCCA lentivirus reprogramming kits (SCR530, SCR531), the Mouse iPS Reprogramming Boost Supplement enhanced the efficiency of mouse iPS colony formation by at least 3-fold. Mouse iPS Reprogramming Boost Supplement can be used to enhance reprogramming efficiency in somatic cell lines that may be more refractory to reprogramming. Mouse iPS cells generated through use of the Mouse iPS Reprogramming Boost Supplement display characteristic ES cell-like morphology, stained positive for alkaline phosphatase, expressed the correct mouse ES cell marker phenotype (SSEA-1 and Sox-2) and can be rapidly expanded in normal mouse ES cell culture conditions.
Armazenamento : -20°C
Embalagem : 1X1EA