LC-SPDP (succinimidyl 6-[3(2-pyridyldithio)propionamido]hexanoate)
REF : 803642-50MG
Marca : Sigma-Aldrich
Descrição :
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Categoria : Synthetic Chemistry - Routine Use
Descrição detalhada : The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents - see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.
Fórmula molecula r: C18 H23 O5 N3 S2
Armazenamento : 2-8C
Embalagem : 1X50MG
Fórmula molecula r: C18 H23 O5 N3 S2
Armazenamento : 2-8C
Embalagem : 1X50MG
