Origami 2 Competent Cell Set* – Novagen

Descrição detalhada : Genotypes:Origami 2Delta(ara-leu)7697 DeltalacX74 DeltaphoA PvuII phoR araD139ahpC galE galK rpsL F'[lac+ lacIq pro] gor522::Tn10trxB (StrR, TetR)Origami 2(DE3)Delta(ara-leu)7697 DeltalacX74 DeltaphoA PvuII phoR araD139ahpC galE galK rpsL F'[lac+ lacIq pro] (DE3) gor522::Tn10trxB (StrR, TetR)Origami 2(DE3)pLysSGenotype: Delta(ara-leu)7697 DeltalacX74 DeltaphoA PvuII phoR araD139ahpC galE galK rpsL F'[lac+ lacIq pro] (DE3) gor522::Tn10trxB pLysS (CamR, StrR, TetR)This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form., Origami 2 host strains are K-12derivatives that have mutations in both thethioredoxin reductase (trxB) and glutathionereductase (gor) genes, which greatly enhancedisulfide bond formation in the E. colicytoplasm. The Origami2 strains are kanamycin sensitive; like theoriginal Origami strains, the gor mutation is still selectedfor by tetracycline. To reduce the possibility ofdisulfide bond formation between molecules,strains containing mutations in trxB and gor arerecommended only for the expression of proteinsthat require disulfide bond formation for properfolding.DE3 indicates that the host is a lysogen of lambdaDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
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